In order to study the contact characteristics of the new rail profile and the high-speed EMUs wheel profiles, the profiles matching between LMA, S1002CN, XP55 wheel and 60D, 60N rail in the middle position had been proceeded. The wheel/rail 3D elastic-plastic contact finite element models were established to analyze the wheel/rail Mises stress, contact status and the longitudinal shear stress under different traction conditions. Through the analysis and comparison of the wheel-rail contact calculation results, the conclusion was drawn. When the wheels contact with the 60D or 60N rail in the middle position, the Mises stress of the 60N rail was smaller and closer to the center of the rail profile. After exerting the traction force, the maximum Mises stress points of wheel had a longitudinal deviation from the rail, which became small with the traction force decreasing, while the wheel/rail maximum Mises stress did not change under variety traction. The contact area of the 60N rail was larger and the sticking area was relatively bigger than that of 60D rail. When the train was running, the maximum longitudinal contact shear stress of the 60N rail was less than the stress value of the 60D rail. Over all, the 60N rail profile could improve the wheel/rail interaction and extend the service life of rail.