Magic formula tire model can describe the tire characteristic accurately. In order to get Magic formula tire model of the running tire, the paper used the combination method of Tire Data Fitting Tool module and genetic algorithm to identify the magic formula parameters. Firstly, rough parameters were identified by using Tire Data Fitting Tool module. Then accurate parameters were identified by using genetic algorithm. Tire force based on the Magic formula tire model had a good correlation with the simulation data. The research provided an effective tire model for the dynamics analysis of straddle-type monorail vehicle.