Common localization technologies were introduced briefly, and localization technology based on RSSI applied in CC2431 was focused on. Process of constructing localization and information acquisition system based on WSN was described, which included design of WSN node adopted embedded technology, application development based on ZigBee protocol stack Z-stack and design of humancomputer interaction interface. Flow of data frame in protocol stack interacted in localization was analyzed emphatically. The results showed that the localization and information acquisition system could achieve real-time localization and meet the needs of monitoring of sensor data.