Combined with the line parameters, running condition, operation schedule, and so on, the recovery and utilization ofregeneration braking energy was analyzed in the same power supply section. The multiple vehicles energy-saving optimization model wasbuilt based on the use of regenerative braking energy and the design of timetable. By introducing the immune algorithm, the headway,dwell time and running time were adjusted, and the train operation strategies was optimized. Finally, the energy-saving train operationschedule and multi vehicle energy-saving driving strategy were obtained in the actual line. The simulation data of one power supplysection of Nanning metro line 1 proved that the utilization rate of regenerative braking energy was improved up to 11.4% and the totalenergy consumption of train was reduced up to 16.9% by using the optimized energy-saving schedules, which ensured the punctuality.